Adaptation of the highly productive T7 expression system to Streptomyces lividans.
نویسندگان
چکیده
Streptomyces lividans is a Gram-positive bacterium known for its remarkable secretion efficiency and low extracellular protease activity. In the present work, we adapted the highly productive T7 expression system to S. lividans. A codon-optimized T7 RNA polymerase gene was chromosomally integrated, and a bifunctional T7 expression vector was constructed.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 76 3 شماره
صفحات -
تاریخ انتشار 2010